Dot blot hybridisation of RNA - Molecular Biology

website slot blot apparatus Soak the membrane with water for 10 min before placing it on a dot blot apparatus Cover unused wells on the membrane apparatus NOTE: Handle the membrane slot xo 888 DotSlot Blotting · Slow or No Filtration of the Sample Through the Membrane · Little or No Protein Observed on the Blot · Stained Blot Is Not Uniform

slot blot apparatus theoretically, you can load as much as the slot takes, however blot? thanks for reply~ I do need it for a western blot I  The Bio-Dot SF microfiltration apparatus is assembled according to manufacturer's instructions and samples are applied onto the PVDF membrane HA and NA  Assemble the dot blot apparatus with a pre-wetted nylon membrane Load denatured viral DNA and linearized standard plasmid DNA solutions into the wells

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